Synonym:TSKgel®Boronate-5PWHPLCColumnProperties:
| RelatedCategories | AffinityChromatography,AffinityHPLCColumns,Analytical/Chromatography,HPLC,HPLC&FPLCColumnsforBiopolymerSeparations, |
| mfr.no. | TosohBoronate-5PW,13066 |
| parameter | ≤20%organicsolvent |
| | ≤3Msaltconcentration |
| | 1.2mL/minflowrate |
| | 30barmax.pressure |
| | ≥0°Ctemperature |
| application(s) | HPLC:suitable |
| L×I.D. | 7.5 cm×7.5 mm |
| matrix | polymerparticleplatform |
| matrixactivegroup | m-aminophenylboronatephase |
| particlesize | 10 μm |
| poresize | 1000 ? |
| operatingpH | 2-9 |
| capacity | 40 μmol/mLadsorptioncapacity(sorbitol) |
| compatibility | modeofuseaffinityHPLC |
| modeofchromatography | Affinity |
| storagetemp. | 2-8°C |
Description:
Generaldescription
Highefficiency,resin-basedTSKgelaffinitycolumns(10μmparticles)separateorpurifymanyenzymesandotherproteins.
Couplingofm-aminophenylboronatetoTSKgel5PWpolymericsupportresultsinaligandcapableofformingatetrahedralboronateanionunderalkalinepHconditions.Thisanionicstructurecanbindwith1,2cis-diolgroupssuchasthosefoundincarbohydrates,carbohydratecontainingcompoundsandcatecholamines.Interactionbetweentheboronateanionandthe1,2cis-diolgroupsisenhancedinthepresenceofMg2+ionsandisinhibitedbyamine-containingbuffers.AdsorptionontotheTSKgelBoronate-5PWmatrixtakesplaceinbasicbufferssuchasHEPESandmorpholinewhiledesorptiontakesplaceincarbohydrateoramine-containingmobilephaselikesorbitolorTris.
Applicationsinclude:nucleicacids,catecholamines,nucleosidesandnucleotides.
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LegalInformation
TSKgelisaregisteredtrademarkofTosohCorporation
Safety:
RIDADR
NONHforallmodesoftransport
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