Synonym:TSKgel®ReversedPhaseHPLCColumn(Polymer-based)Properties:
| RelatedCategories | Analytical/Chromatography,HPLC,HPLC&FPLCColumnsforBiopolymerSeparations,TSKgelHPLCColumns,TOYOPEARLBulkMedia&Accessories,TSKgelPhenyl-5PWRPColumns(1000?pores), |
| material | stainlesssteelhardware |
| mfr.no. | TosohPhenyl-5PW,16260 |
| extentoflabeling | 1 meq/mLliganddensity(estimate) |
| parameter | saltconcentration |
| | 0-100%organicsolvent |
| | 30barmax.pressure |
| | 4-60°Ctemperature |
| | 8mL/minflowrate |
| application(s) | HPLC:suitable |
| L×I.D. | 15 cm×21.5 mm |
| matrix | polymerparticleplatform |
| matrixactivegroup | phenylphase |
| particlesize | 13 μm |
| poresize | 1000 ? |
| operatingpH | 2-12 |
| modeofchromatography | Reversedphase |
Description:
Generaldescription
Polymer-basedparticlesforreversed-phase(RP)HPLCgenerallyhavetwobenefitsoversilica-basedparticles:TheyaremoretolerantofhighandlowpHoperatingconditionsandtheydonothavethesilanolinteractionsthatcausepeaktailingofsomebasicanalytes.Also,somesilicashaveanintrinsicmetalcontentthatmaycausechelationproblemswithsensitivebiomolecules,leADIngtopoorrecovery.PolymericTSKgelcolumnscomeinC18andphenylfunctionalgroupsandarangeofparticleporosities.Fastkineticsandquantitativeproteinrecoveryatsubmicrogramloadingarecharacteristicofnonporousresin-basedTSKgelOctadecyl-NPRcolumns.
• Octadecyl-2PWparticleshave125?pores
• Octadecyl-4PWparticleshave500?pores
• Phenyl-5PWRPparticleshave1000?pores
• Octadecyl-NPRparticlesarenonporous
TSKgelPhenyl-5PWRPofferssimiliarselectivitytoitshydrophobicinterationcounterpartTSKgelPhenyl-5PW,althoughahigherdegreeofcross-linkingallowsforincreasedstABIlityinaqueous/organicmobilephasegradients.Thehighlycross-linkedpolymethacrylatebasematerialprovidesanadvantageoversilicawhenhighpHbuffersystemsareneeded.Additionally,TSKgelPhenyl-5PWRPcanwithstandrigorouscleaningprotocolsusingeitheracidorbase.The1000?poresizeaccommodatesglobularproteinsamplesupto1,000,000Da.
Physicalform
Shippedin30%methanol-70%water.
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LegalInformation
TSKgelisaregisteredtrademarkofTosohCorporation
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