Synonym:TSKgel®ReversedPhaseHPLCColumn(Polymer-based)Properties:
| RelatedCategories | Analytical/Chromatography,HPLC,HPLC&FPLCColumnsforBiopolymerSeparations,TSKgelHPLCColumns,TOYOPEARLBulkMedia&Accessories,TSKgelOctadecyl-2PWColumns(125?pores), |
| material | stainlesssteelhardware |
| mfr.no. | Tosoh,17500 |
| extentoflabeling | 1 meq/mLliganddensity(estimate) |
| parameter | 0-100%organicsolvent |
| | 1.2mL/minflowrate |
| | 120barmax.pressure |
| | 4-50°Ctemperature |
| application(s) | HPLC:suitable |
| L×I.D. | 15 cm×4.6 mm |
| matrix | polymerparticleplatform |
| matrixactivegroup | C18(octadecyl)phase |
| particlesize | 5 μm |
| poresize | 125 ? |
| operatingpH | 2-12 |
| modeofchromatography | Reversedphase |
Description:
Generaldescription
Polymer-basedparticlesforreversed-phase(RP)HPLCgenerallyhavetwobenefitsoversilica-basedparticles:TheyaremoretolerantofhighandlowpHoperatingconditionsandtheydonothavethesilanolinteractionsthatcausepeaktailingofsomebasicanalytes.Also,somesilicashaveanintrinsicmetalcontentthatmaycausechelationproblemswithsensitivebiomolecules,leADIngtopoorrecovery.PolymericTSKgelcolumnscomeinC18andphenylfunctionalgroupsandarangeofparticleporosities.Fastkineticsandquantitativeproteinrecoveryatsubmicrogramloadingarecharacteristicofnonporousresin-basedTSKgelOctadecyl-NPRcolumns.
• Octadecyl-2PWparticleshave125?pores
• Octadecyl-4PWparticleshave500?pores
• Phenyl-5PWRPparticleshave1000?pores
• Octadecyl-NPRparticlesarenonporous
Highlycross-linkedpolymethacrylatebasematerialofTSKgelOctadecyl-2PWprovidesexcellentstABIlityinhighpHbuffersystemsandcanwithstandrigorouscleaningwitheitheracidorbase.The125?poresallowforanaylsisofpeptidesupto8,000Da.Columnspackedwithlargerparticlesizesareavailableforsemi-preparativescaleseparations.
Physicalform
Shippedin55%acetonitrile-45%water.
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LegalInformation
TSKgelisaregisteredtrademarkofTosohCorporation
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