Synonym:TSKgel®Amide-80,5cmx4.6mmID,5μmProperties:
RelatedCategories | 5and10μmTSKgelAmide-80andAmide-80HRColumns,Analytical/Chromatography,HPLC,HPLC&FPLCColumnsforBiopolymerSeparations,TSKgelHILICHPLCColumns, |
material | stainlesssteelhardware |
mfr.no. | Tosoh,19532 |
parameter | 0-100%organicsolvent |
| 10-80°Ctemperature |
| 150barmax.pressure |
application(s) | HPLC:suitable |
L×I.D. | 5 cm×4.6 mm |
matrix | silicaparticleplatform |
matrixactivegroup | carbamoylphase |
particlesize | 5 μm |
poresize | 100 ? |
surfacearea | 450 m2/g |
operatingpH | 2.0-7.5 |
suitABIlity | suitableforL68perUSP |
modeofchromatography | Hydrophilicinteraction(HILIC) |
Description:
Generaldescription
TSKgelAmide-80columnsarepackedwith3,5,or10micronsphericalsilicaparticlesthatarecovalentlybondedwithcarbamoylgroups.Theamidestationaryphaseprovidesauniqueselectivityunderregularnormalphaseconditionsorinthehydrophilicinteractionmodeofchromatography.Targetapplicationsincludetheanalysisofsaccharides,glycans,oligosaccarides,peptidesandpolarcompoundsfromnaturalproducts.UniqueadvantagesofAmide-80phaseforsaccharideanalysisincludenovelhydrogenbondingretentionmechanismbetweenhydroxylgroupsofthesampleandthecarbamoylgroupinthestationaryphase.Thestationaryphasedoesnotreactwithreducingsugarsandcanbeusedatelevatedtemperatures(4to80°C)topreventpeaksplittingofcarbohydratesthatcanoccuratlowertemperatures.Inaddition,thenew,highefficiency,3microncolumnsoffershorteranalysistimeandenhancedsensitivityforLC-MSapplications.
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LegalInformation
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