Synonym:TSKgel®Amide-80,25cmx2mmID,5μmProperties:
| RelatedCategories | 5and10μmTSKgelAmide-80andAmide-80HRColumns,Analytical/Chromatography,HPLC,HPLC&FPLCColumnsforBiopolymerSeparations,TSKgelHILICHPLCColumns, |
| material | stainlesssteelhardware |
| mfr.no. | Tosoh,19697 |
| parameter | 0-100%organicsolvent |
| | 10-80°Ctemperature |
| | 150barmax.pressure |
| application(s) | HPLC:suitable |
| L×I.D. | 25 cm×2 mm |
| matrix | silicaparticleplatform |
| matrixactivegroup | carbamoylphase |
| particlesize | 5 μm |
| poresize | 100 ? |
| surfacearea | 450 m2/g |
| operatingpH | 2.0-7.5 |
| suitABIlity | suitableforL68perUSP |
| modeofchromatography | Hydrophilicinteraction(HILIC) |
Description:
Generaldescription
TSKgelAmide-80columnsarepackedwith3,5,or10micronsphericalsilicaparticlesthatarecovalentlybondedwithcarbamoylgroups.Theamidestationaryphaseprovidesauniqueselectivityunderregularnormalphaseconditionsorinthehydrophilicinteractionmodeofchromatography.Targetapplicationsincludetheanalysisofsaccharides,glycans,oligosaccarides,peptidesandpolarcompoundsfromnaturalproducts.UniqueadvantagesofAmide-80phaseforsaccharideanalysisincludenovelhydrogenbondingretentionmechanismbetweenhydroxylgroupsofthesampleandthecarbamoylgroupinthestationaryphase.Thestationaryphasedoesnotreactwithreducingsugarsandcanbeusedatelevatedtemperatures(4to80°C)topreventpeaksplittingofcarbohydratesthatcanoccuratlowertemperatures.Inaddition,thenew,highefficiency,3microncolumnsoffershorteranalysistimeandenhancedsensitivityforLC-MSapplications.
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LegalInformation
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