Properties:
| RelatedCategories | Analytical/Chromatography,HPLC,HPLC&FPLCColumnsforBiopolymerSeparations,TSKgelHPLCColumns,TOYOPEARLBulkMedia&Accessories,ToyoScreenHydrophobicInteractionProcessDevelopmentColumns, |
| material | aluminumseal |
| | polypropyleneandpolyethylenefrit |
| packaging | pkgof6×5 mL |
| mfr.no. | Tosoh,21373 |
| parameter | ≤50%organicsolvent |
| | 1-5mL/minflowrate |
| | 20mL/minmax.flowrate |
| | 3barmax.pressure |
| L×I.D. | 3 cm×14.6 mm |
| matrix | hydroxylatedmethacrylicpolymer |
| matrixactivegroup | etherphase |
| particlesize | 65 μm |
| operatingpH | 1-13 |
| capacity | 20 g/L±10adsorptioncapacity(lysozyme) |
| modeofchromatography | Hydrophobicinteraction(HIC) |
Description:
Generaldescription
Everylargescalecommercialpurificationbeginsatasmallscaleinamethoddevelopmentlabbyevaluatingretentionandselectivityofthetargetproteinversusimpurities,hostcellproteins,DNA,etc.,onmultipleresins,oftenfromdifferentvendors.Dependingontheconstraints,theprocessdevelopmentengineercaneitherpurchasebulkresinstopackseveralsmallcolumnswithdifferentresinsortakeadvantageofthespeedandconvenienceofpre-packedcolumnsforresinevaluation.Pre-packedToyoScreen®ProcessDevelopmentColumnsareavailablein1mLand5mLvolumespackedwiththemostpopularToyopearl®resins.ToyoScreencolumnscanbeusedtoquicklyestablishoptimumelutionconditions.VaryingpH,salttype,grADIentconditionsandflowratearecommonexperimentalparametersexplored.InitialresultsfromresinscreeningandoptimizationwithToyoScreencolumnsaccuratelypredicttheseparationbehavioratlargerscale.
Recommendedproducts
DiscoverLiChropurreagentsidealforHPLCorLC-MSanalysis
Specifications
Cleaninplacewith0.5MNaOHor0.1MHCl.
LegalInformation
ToyoScreenisaregisteredtrademarkofTosohCorporation
ToyopearlisaregisteredtrademarkofTosohCorporation
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