Synonym:TOYOPEARL®AF-rProteinA-650FBulkMediaProperties:
| RelatedCategories | Affinity,Analytical/Chromatography,HPLC,HPLC&FPLCColumnsforBiopolymerSeparations,TOYOPEARLBulkChromatographyMedia, |
| description | Adsorptioncapacity(>45g/LIgG(static);>30g/LIgG(dynamicat2min.residencetime)) |
| | CleaninPlace(CIP)(0.1MNaOH) |
| | Phasefilter(ProteinA) |
| | Shippingsolvent(20%(v/v)ethanol) |
| form | 72%(v/v)slurryin20%(v/v)ethanol |
| packaging | bottleof1 L |
| mfr.no. | Tosoh,22806 |
| parameter | 3barmax.pressure |
| matrix | hydroxylatedmethacrylicpolymerparticleplatform |
| matrixactivegroup | rProteinAphase |
| particlesize | 45 μm |
| poresize | 1000 ? |
| pHrange | 2-13 |
| capacity | >30 g/Ladsorptioncapacity(IgG)(dynamicat2min.residencetime) |
| | >45 g/Ladsorptioncapacity(IgG)(static) |
| modeofchromatography | Affinity |
Description:
Generaldescription
ProteinAchromatography,themostwidelyusedtypeofaffinitychromatography,reliesonthespecificandreversIBLebindingofantibodiestoanimmobilizedligand;inthiscaseproteinA.ProteinAisa56kDasurfaceproteinnativetothecellwallofthebacteriumStaphylococcusaureus.Itiscomposedoffiveimmunoglobulin-bindingdomains,eachofwhichareabletobindproteinsfrommanymammalianspecies,mostnotablyImmunoglobulinG(IgG)throughtheheavychainwithintheFcregion.WhilethenativeformofProteinAwasusedastheligandforfirstgenerationProteinAresins,therecombinantform(rProteinA)producedinE.coliisthemostprevalenttoday.Modificationstotheproteinstructureoftheligand,theadventofligandscomposedofsingledomainmultimers,andmultipointattachmenthavegivenrisetothecausticstable,highcapacityandextremelyrobustProteinAresinsinusetoday.
TheproteinAligandcaneitherbinddirectlytotheFcregionofanantibodyortoanFctagthathasbeenfusedtothetargetofinterest.ProteinAchromatographyisaveryrobustpurificationprocedureandisusedasacapturestepduetoitsspecificityand,dependingontheintendeduseforthetargetmolecule(antibodiesfordiagnostictesting),mightbetheonlychromatographicsteprequiredtoachieveadequateproductpurity.ToyopearlAF-rProteinA-650FutilizesanewalkalineresistantrecombinantProteinAvariant.Thisnewnon-animalderivedligandisboundtothebasebead,ToyopearlHW-65F,usingmultipointattachment.Thiscontributestoboththenewresin′salkalineresistanceanditslowligandleakagesintherange5-25ng/mgIgG.
ToyopearlAF-rProteinA-650FmaintainsitscapacitywellafterrepeatedCIPexposureto0.1mol/LNaOHandhasreasonabletoleranceto0.5mol/LNaOHCIP.
Thenewresinalsotoleratesexposuretoaboveambienttemperaturesandifneededcanbeautoclaved.
Goodpressure-flowcharacteristicsandkineticscontributetomaintaininggoodcapacityparticularlyatlowresidencetimes.
Physicalform
Shippedin20%(v/v)ethanol.
Recommendedproducts
DiscoverLiChropurreagentsidealforHPLCorLC-MSanalysis
Specifications
Cleaninplacewith0.1MNaOH.
LegalInformation
ToyopearlisaregisteredtrademarkofTosohCorporation
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