Properties:
| Related Categories |
Analytical/Chromatography,
HPLC,
HPLC & FPLC Columns for Biopolymer Separations,
TSKgel HPLC Columns, TOYOPEARL Bulk Media & Accessories,
TSKgel Protein A HPLC Column
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Description:
General description
TSKgel Protein A-5PW is a 20 μm, 4.6 mm ID × 3.5 cm column for high performance affinity chromatography. Made of PEEK hardware, this column has been designed for the rapid separation and robust quantification of a
variety of antibodies. Monoclonal antibodies from harvested cell culture media can be captured and accurately quantitated in less than 2 minutes per injection.
The TSKgel Protein A-5PW column can be used for more than 2,000 injections without regeneration or cleaning. Packed with hydroxylated methacrylic polymer beads, this column is designed with a high degree
of crosslinking, which allows a high flow rate for chromatography while still maintaining chromatographic efficiency, peak width and resolution. The recombinant protein A ligand is a code-modified hexamer of the C domain.
An enhanced rProtein A ligand is bound to the TSKgel 5PW base bead via multipoint attachment resulting in excellent base stability in 0.1 mol/L NaOH.
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Footnote
Highlights
• Wide dynamic range with high sample concentration load for mAb quantitation
• Fast analysis: 1-2 min/analysis
• High sensitivity for mAb determination
• Long lifetime: .2,000 injections per column with no sign of deterioration
Legal Information
TSKgel is a registered trademark of Tosoh Corporation