Isoelectric Focusing and Two Dimensional Electrophoresis,
Molecular Biology,
Physical Properties Standards,
Protein Electrophoresis,
Proteomics,
Stock Solution
More...
concentration
1 mg/mL in 10 mM HCl
fluorescence
λex 385 nm; λem 495 nm in 0.1 M phosphate pH 7.6
storage temp.
2-8°C
Description:
Application
Suitable as IEF-Marker for HPCE and for IEF-gels
Preparation Note
Filtered through a 0.45 μm membrane filter.
Reconstitution
For use in HPCE the marker should be diluted 1:100 in a suitable ampholyte solution (e.g. 2% ampholyte 3-10), UV-detection at 280 nm (20 °C). For use in IEF-gels the solution can be loaded directly onto the gel (max. 1 μl per lane).