Synonym:
TCA
Properties:
| Related Categories |
Acids,
Acids & Bases,
Acids and Bases,
Analytical Reagents,
Analytical Reagents for General Use,
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grade
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ACS reagent
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vapor density
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<1 (vs air)
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vapor pressure
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1 mmHg ( 51 °C)
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InChI Key
|
YNJBWRMUSHSURL-UHFFFAOYSA-N
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assay
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≥99.5% (T)
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≥99.5%
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quality
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for the determination of Fe in blood according to Heilmeyer
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ign. residue
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≤0.03% (as SO4)
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refractive index
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n20/D 1.62(lit.)
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bp
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196 °C(lit.)
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mp
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54-58 °C(lit.)
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solubility
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water: soluble 81.7 g/L at 20 °C
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density
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1.62 g/mL at 25 °C(lit.)
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anion traces
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chloride (Cl-): ≤10 mg/kg
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nitrate (NO3-): ≤20 mg/kg
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phosphate (PO43-): ≤5 mg/kg
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sulfate (SO42-): ≤200 mg/kg
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cation traces
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Ca: ≤10 mg/kg
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Cd: ≤5 mg/kg
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Co: ≤5 mg/kg
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Cr: ≤5 mg/kg
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Cu: ≤5 mg/kg
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Fe: ≤5 mg/kg
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K: ≤50 mg/kg
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Mg: ≤5 mg/kg
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Mn: ≤5 mg/kg
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Na: ≤50 mg/kg
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Ni: ≤5 mg/kg
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Pb: ≤5 mg/kg
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Zn: ≤5 mg/kg
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Description:
Application
Traditionally used to precipitate protein. Has been used to determine protein concentration by quantitative precipitation. Used as decalcifier and fixative in microscopy.
Trichloroacetic acid may be used in the following studies:
• Estimation of phosphorus in various biological samples (serum, urine and stool ash solutions).
• Elimination of acid-soluble and lipid phosphorous during the determination of deoxyribonucleic acid, ribonucleic acid and phosphoproteins in animal tissues.
• Preparation of 2-methyl-4-nitroanilinium trichloroacetate trichloroacetic acid (2M4NATCA) crystals.
10% Trichloroacetic acid may be employed for the preparation of radioactive proteins.
Other Notes
Protein denaturant
General description
Trichloroacetic acid (TCA) is a chlorinated acetic acid. It is one of the major metabolites of trichloroethylene (TCE).
Safety:
Hazard statements
H314-H335-H410
Precautionary statements
P260-P280-P303 + P361 + P353-P304 + P340 + P310-P305 + P351 + P338
Personal Protective Equipment
Eyeshields, Faceshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges