Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
| ProductName | 14-3-3zeta(Phospho-Ser58)Antibody |
| Synonyms | 14-3-3proteinzeta/delta;1433Z;143Z;FAS;FactoractivatingexoenzymeS;KCIP-1;MitochondrialimportstimulationfactorS1subunit;ProteinkinaseCinhibitorprotein-1;YWHAZ |
| Immunogen | Theantiserumwasproducedagainstsynthesizedpeptidederivedfromhuman14-3-3zetaaroundthephosphorylationsiteofSer58. |
| ImmunogenRange | 24-73 |
| Clonality | Polyclonal |
| ApplicationDilutions | WB:1:500~1:1000IHC:1:50~1:100IF:1:100~1:500ELISA:1:40000 |
| Specificity | 14-3-3zeta(Phospho-Ser58)Antibodydetectsendogenouslevelsof14-3-3zetaonlywhenphosphorylatedatSer58. |
| Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
| PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
| Storage/StABIlity | Stableat-20°Cforatleast1year. |
| NCBIGeneSymbol | YWHAZ |
| DatabaseLinks | GeneID: 7534 Swiss-Prot#: P63104 OMIM#: 601288 Unigene#: Hs.492407/Hs.594673 |
| TargetModification | Phospho |
| ModificationSites | H:S58M:S58R:S58 |
| ApplicationImages |
| Image1 | ImmunofluorescenceanalysisofHeLacellstreatedwithPMA125ng/ml30",using14-3-3zeta(Phospho-Ser58)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
| Image2 |  |
| Image3 | WesternblotanalysisoflysatesfromNIH/3T3cellstreatedwithUV30",using14-3-3zeta(Phospho-Ser58)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |