Product Name
AMPK alpha 1/2 (Phospho-Thr183/172) Antibody
Catalog No
A0003
Storage/Stability
Stable at -20°C for at least 1 year.
Applications
WB, IHC, ELISA
Clonality
Polyclonal
Host Species
Rabbit
Reactivity
Human, Mouse, Rat
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000
Database Links
Gene ID: 5562/5563, Swiss-Prot #: Q13131/P54646, OMIM #: 602739/600497, Unigene #: Hs.43322/Hs.437039
Immunogen
The antiserum was produced against synthesized peptide derived from human AMPK alpha 1/2 around the phosphorylation site of Thr183/172.
Immunogen Range
140-189
Modification Sites
H: T183/172 M: T183/172 R: T183/172
MW
62 kDa
NCBI Gene Symbol
PRKAA1, PRKAA2
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Specificity
AMPK alpha 1/2 (Phospho-Thr183/172) Antibody detects endogenous levels of AMPK alpha 1/2 only when phosphorylated at Thr183/172.
Target Modification
Phospho