Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
ProductName | IkappaB-epsilon(Phospho-Ser22)Antibody |
Synonyms | I-kappa-B-epsilon;IKBE;IkappaBepsilon;NF-kappa-BIE;NF-kappaBinhibitorepsilon;NFKBIE |
Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanIkappaB-epsilonaroundthephosphorylationsiteofSer22. |
ImmunogenRange | 131-180 |
Clonality | Polyclonal |
ApplicationDilutions | WB:1:500~1:1000IHC:1:50~1:100IF:1:100~1:500ELISA:1:40000 |
Specificity | IkappaB-epsilon(Phospho-Ser22)AntibodydetectsendogenouslevelsofIkappaB-epsilononlywhenphosphorylatedatSer22. |
Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
Storage/StABIlity | Stableat-20°Cforatleast1year. |
NCBIGeneSymbol | NFKBIE |
DatabaseLinks | GeneID: 4794 Swiss-Prot#: O00221 OMIM#: 604548 Unigene#: Hs.458276 |
TargetModification | Phospho |
ModificationSites | H:S22M:S22R:S22 |
ApplicationImages |
Image1 | ImmunofluorescenceanalysisofHeLacells,usingIkappaB-epsilon(Phospho-Ser22)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image2 | Immunohistochemistryanalysisofparaffin-embeddedhumanbreastcarcinoma,usingIkappaB-epsilon(Phospho-Ser22)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image3 | WesternblotanalysisoflysatesfromJurkatcellstreatedwithTNF-a20ng/ml30",usingIkappaB-epsilon(Phospho-Ser22)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |