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| Note: The product is for research use only, not for use in diagnostic or therapeutic procedures |
|
| Product Name | PKC zeta (Phospho-Thr410) Antibody |
| Synonyms | KPCZ; PKC-zeta; PKC2; PRKCZ; Protein kinase C zeta type; kinase PKC-zeta; nPKC-zeta |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PKC zeta around the phosphorylation site of Thr410. |
| Immunogen Range | 376-425 |
| Clonality | Polyclonal |
| Application Dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000 |
| Specificity | PKC zeta (Phospho-Thr410) Antibody detects endogenous levels of PKC zeta only when phosphorylated at Thr410. |
| Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
| Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage/Stability | Stable at -20°C for at least 1 year. |
| NCBI Gene Symbol | PRKCZ |
| Database Links |
Gene ID: 5590 Swiss-Prot #: Q05513 OMIM #: 176982 Unigene #: Hs.496255 |
| Target Modification | Phospho |
| Modification Sites | H:T410 M:T410 R:T410 |
| Application Images | |
| Image 1 |
 Immunofluorescence analysis of HeLa cells, using PKC zeta (Phospho-Thr410) Antibody. The picture on the right is blocked with the phospho peptide. |
| Image 2 |
 Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using PKC zeta (Phospho-Thr410) Antibody. The picture on the right is blocked with the phospho peptide. |
| Image 3 |
 Western blot analysis of lysates from NIH/3T3 cells treated with PMA 125ng/ml 30', using PKC zeta (Phospho-Thr410) Antibody. The lane on the right is blocked with the phospho peptide. |
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