Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
ProductName | SHP-2(Phospho-Tyr580)Antibody |
Synonyms | PTN11;PTP-1D;PTP-2C;PTP2C;PTPN11;Protein-tyrosinephosphatase2C;Protein-tyrosinephosphatase;non-receptortype11;SH-PTP2;SH-PTP3;SHPTP2 |
Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanSHP-2aroundthephosphorylationsiteofTyr580. |
ImmunogenRange | 546-595 |
Clonality | Polyclonal |
ApplicationDilutions | WB:1:500~1:1000IHC:1:50~1:100ELISA:1:40000 |
Specificity | SHP-2(Phospho-Tyr580)AntibodydetectsendogenouslevelsofSHP-2onlywhenphosphorylatedatTyr580. |
Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
Storage/StABIlity | Stableat-20°Cforatleast1year. |
NCBIGeneSymbol | PTPN11 |
DatabaseLinks | GeneID: 5781 Swiss-Prot#: Q06124 OMIM#: 151100/163950/163955/176876/607785 Unigene#: Hs.506852/Hs.646231 |
TargetModification | Phospho |
ModificationSites | H:Y580M:Y584R:Y580 |
ApplicationImages |
Image1 | Immunohistochemistryanalysisofparaffin-embeddedhumanbrain,usingSHP-2(Phospho-Tyr580)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image2 | WesternblotanalysisoflysatesfromA431cells,usingSHP-2(Phospho-Tyr580)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |