The antiserum was produced against synthesized peptide derived from human Synaptotagmin around the phosphorylation site of Ser309.
Immunogen Range
276-325
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
Synaptotagmin (Phospho-Ser309) Antibody detects endogenous levels of Synaptotagmin only when phosphorylated at Ser309.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Synaptotagmin (Phospho-Ser309) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from 293 cells treated with Sobital 0.4M 30', using Synaptotagmin (Phospho-Ser309) Antibody. The lane on the right is blocked with the phospho peptide.