The antiserum was produced against synthesized peptide derived from human CDC25B around the phosphorylation site of Ser323.
Immunogen Range
289-338
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:20000
Specificity
CDC25B (Phospho-Ser323) Antibody detects endogenous levels of CDC25B only when phosphorylated at Ser323.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
CDC25A
Database Links
Gene ID: 994 Swiss-Prot #: P30305
OMIM #: 116949 Unigene #: Hs.437705
Target Modification
Phospho
Modification Sites
H:S323 M:S321 R:S319
Application Images
Image 1
Western blot analysis of lysates from NIH/3T3 cells treated with PMA 125ng/ml 30', using CDC25B (Phospho-Ser323) Antibody. The lane on the right is blocked with the phospho peptide.