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| Note: The product is for research use only, not for use in diagnostic or therapeutic procedures |
|
| Product Name | COT (Phospho-Thr290) Antibody |
| Synonyms | C-COT; COT proto-oncogene serine/threonine-protein kinase; Cancer Osaka thyroid oncogene; M3K8; MAP3K8; Mitogen-activated protein kinase kinase kinase 8; TPL-2; TPL2; Tpl2; Tumor progression locus 2; kinase Cot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human COT around the phosphorylation site of Thr290. |
| Immunogen Range | 256-305 |
| Clonality | Polyclonal |
| Application Dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000 |
| Specificity | COT (Phospho-Thr290) Antibody detects endogenous levels of COT only when phosphorylated at Thr290. |
| Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
| Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage/Stability | Stable at -20°C for at least 1 year. |
| NCBI Gene Symbol | MAP3K8 |
| Database Links |
Gene ID: 1326 Swiss-Prot #: P41279 OMIM #: 191195 Unigene #: Hs.432453 |
| Target Modification | Phospho |
| Modification Sites | H:T290 M:T290 R:T290 |
| Application Images | |
| Image 1 |
 Immunofluorescence analysis of HUVEC cells, using COT (Phospho-Thr290) Antibody. The picture on the right is blocked with the phospho peptide. |
| Image 2 |
 Immunohistochemistry analysis of paraffin-embedded human brain, using COT (Phospho-Thr290) Antibody. The picture on the right is blocked with the phospho peptide. |
| Image 3 |
 Western blot analysis of lysates from 293 cells treated with UV 15', using COT (Phospho-Thr290) Antibody. The lane on the right is blocked with the phospho peptide. |
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