Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
ProductName | CrkII(Phospho-Tyr221)Antibody |
Synonyms | Adaptermoleculecrk;CRKO;Crk-II;P38;Proto-oncogeneC-crk |
Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanCrkIIaroundthephosphorylationsiteofTyr221. |
ImmunogenRange | 187-236 |
Clonality | Polyclonal |
ApplicationDilutions | WB:1:500~1:1000IF:1:100~1:500ELISA:1:40000 |
Specificity | CrkII(Phospho-Tyr221)AntibodydetectsendogenouslevelsofCrkIIonlywhenphosphorylatedatTyr221. |
Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
Storage/StABIlity | Stableat-20°Cforatleast1year. |
NCBIGeneSymbol | CRK |
DatabaseLinks | GeneID: 1398 Swiss-Prot#: P46108 OMIM#: 164762 Unigene#: Hs.638121 |
TargetModification | Phospho |
ModificationSites | H:Y221M:Y221R:Y221 |
ApplicationImages |
Image1 | ImmunofluorescenceanalysisofHUVECcells,usingCrkII(Phospho-Tyr221)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image2 | WesternblotanalysisoflysatesfromCOS7cells,usingCrkII(Phospho-Tyr221)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |