Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
ProductName | CrkL(Phospho-Tyr207)Antibody |
Synonyms | CRKOL;Crk-likeprotein |
Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanCrkLaroundthephosphorylationsiteofTyr207. |
ImmunogenRange | 173-222 |
Clonality | Polyclonal |
ApplicationDilutions | WB:1:500~1:1000IHC:1:50~1:100IF:1:100~1:500ELISA:1:5000 |
Specificity | CrkL(Phospho-Tyr207)AntibodydetectsendogenouslevelsofCrkLonlywhenphosphorylatedatTyr207. |
Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
Storage/StABIlity | Stableat-20°Cforatleast1year. |
NCBIGeneSymbol | CRKL |
DatabaseLinks | GeneID: 1399 Swiss-Prot#: P46109 OMIM#: 602007 Unigene#: Hs.592205 |
TargetModification | Phospho |
ModificationSites | H:Y207M:Y207R:Y207 |
ApplicationImages |
Image1 | ImmunofluorescenceanalysisofHUVECcells,usingCrkL(Phospho-Tyr207)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image2 | Immunohistochemistryanalysisofparaffin-embeddedhumanlungcarcinoma,usingCrkL(Phospho-Tyr207)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image3 | WesternblotanalysisoflysatesfromCOS7cells,usingCrkL(Phospho-Tyr207)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |