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| Note: The product is for research use only, not for use in diagnostic or therapeutic procedures |
|
| Product Name | eEF2K (Phospho-Ser366) Antibody |
| Synonyms | Calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase; EF2K; Elongation factor 2 kinase; eEF-2 kinase; eEF-2K; kinase eEF2K |
| Immunogen | The antiserum was produced against synthesized peptide derived from human eEF2K around the phosphorylation site of Ser366. |
| Immunogen Range | 331-380 |
| Clonality | Polyclonal |
| Application Dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000 |
| Specificity | eEF2K (Phospho-Ser366) Antibody detects endogenous levels of eEF2K only when phosphorylated at Ser366. |
| Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
| Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage/Stability | Stable at -20°C for at least 1 year. |
| NCBI Gene Symbol | EEF2K |
| Database Links |
Gene ID: 29904 Swiss-Prot #: O00418 OMIM #: 606968 Unigene #: Hs.633744 |
| Target Modification | Phospho |
| Modification Sites | H:S366 R:S365 |
| Application Images | |
| Image 1 |
 Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using eEF2K (Phospho-Ser366) Antibody. The picture on the right is blocked with the phospho peptide. |
| Image 2 |
 Western blot analysis of lysates from HeLa cells treated with serum 10% 15', using eEF2K (Phospho-Ser366) Antibody. The lane on the right is blocked with the phospho peptide. |
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