Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
ProductName | Abl(Phospho-Tyr412)Antibody |
Synonyms | ABL1;JTK7;Proto-oncogenetyrosine-proteinkinaseABL1;c-ABL;p150 |
Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanAblaroundthephosphorylationsiteofTyr412. |
ImmunogenRange | 361-410 |
Clonality | Polyclonal |
ApplicationDilutions | WB:1:500~1:1000IHC:1:50~1:100ELISA:1:1000 |
Specificity | Abl(Phospho-Tyr412)AntibodydetectsendogenouslevelsofAblonlywhenphosphorylatedatTyr412. |
Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
Storage/StABIlity | Stableat-20°Cforatleast1year. |
NCBIGeneSymbol | ABL1 |
DatabaseLinks | GeneID: 25/27 Swiss-Prot#: P00519/P42684 OMIM#: 189980/608232/164690 Unigene#: Hs.431048/Hs.159472 |
TargetModification | Phospho |
ModificationSites | H:Y393/439M:Y393/439 |
ApplicationImages |
Image1 | Immunohistochemistryanalysisofparaffin-embeddedhumanlungcarcinoma,usingAbl(Phospho-Tyr412)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image2 | WesternblotanalysisoflysatesfromCOS7cellstreatedwithAdriamycin0.5ug/ml24h,usingAbl(Phospho-Tyr412)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |