Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
ProductName | E2F1(Phospho-Thr433)Antibody |
Synonyms | E2F-1;PBR3;PRB-bindingproteinE2F-1;RBAP-1;RBBP-3;RBBP3;Retinoblastomabindingprotein3;Retinoblastoma-associatedprotein1;TranscriptionfactorE2F1 |
Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanE2F1aroundthephosphorylationsiteofThr433. |
ImmunogenRange | 388-437 |
Clonality | Polyclonal |
ApplicationDilutions | WB:1:500~1:1000ELISA:1:5000 |
Specificity | E2F1(Phospho-Thr433)AntibodydetectsendogenouslevelsofE2F1onlywhenphosphorylatedatThr433. |
Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
Storage/StABIlity | Stableat-20°Cforatleast1year. |
NCBIGeneSymbol | E2F1 |
DatabaseLinks | GeneID: 1869 Swiss-Prot#: Q01094 OMIM#: 189971 Unigene#: Hs.654393 |
TargetModification | Phospho |
ModificationSites | H:T433M:T426 |
ApplicationImages |
Image1 | WesternblotanalysisoflysatesfromHeLacellstreatedwithEtoposide25uM24h,usingE2F1(Phospho-Thr433)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |