Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
ProductName | FAK(Phospho-Tyr576)Antibody |
Synonyms | FADK1;FAK1;Focaladhesionkinase1;pp125FAK;Protein-tyrosinekinase2;PTK2 |
Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanFAKaroundthephosphorylationsiteofTyr576. |
ImmunogenRange | 542-591 |
Clonality | Polyclonal |
ApplicationDilutions | WB:1:500~1:1000IHC:1:50~1:100ELISA:1:1000 |
Specificity | FAK(Phospho-Tyr576)AntibodydetectsendogenouslevelsofFAKonlywhenphosphorylatedatTyr576. |
Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
Storage/StABIlity | Stableat-20°Cforatleast1year. |
NCBIGeneSymbol | PTK2 |
DatabaseLinks | GeneID: 5747 Swiss-Prot#: Q05397 OMIM#: 600758 Unigene#: Hs.395482 |
TargetModification | Phospho |
ModificationSites | H:Y576M:Y614R:Y576 |
ApplicationImages |
Image1 | Immunohistochemistryanalysisofparaffin-embeddedhumanbrain,usingFAK(Phospho-Tyr576)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image2 | WesternblotanalysisoflysatesfromNIH/3T3cells,usingFAK(Phospho-Tyr576)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |