The antiserum was produced against synthesized peptide derived from human IL-3R beta around the phosphorylation site of Tyr593.
Immunogen Range
559-608
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
Specificity
IL-3R beta (Phospho-Tyr593) Antibody detects endogenous levels of IL-3R beta only when phosphorylated at Tyr593.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
CSF2RB
Database Links
Gene ID: 1439 Swiss-Prot #: P32927
OMIM #: 138981/265120 Unigene #: Hs.592192
Target Modification
Phospho
Modification Sites
H:Y593 M:Y595 R:Y595
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human brain, using IL-3R beta (Phospho-Tyr593) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from RAW264.7 cells treated with G-CSF 25ng/ml 15', using IL-3R beta (Phospho-Tyr593) Antibody. The lane on the right is blocked with the phospho peptide.