Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
ProductName | ATPase(Phospho-Ser16)Antibody |
Synonyms | A1A1;AT1A1;ATPase,Na+/K+transporting,alpha1polypeptide;HypotheticalproteinMGC38419;Na+/K+ATPase1;Na,K-ATPase1;Sodiumpump1;Sodium/potassium-transportingATPasealpha-1chainprecursor |
Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanATPasearoundthephosphorylationsiteofSer16. |
ImmunogenRange | May-54 |
Clonality | Polyclonal |
ApplicationDilutions | WB:1:500~1:1000IHC:1:50~1:100ELISA:1:1000 |
Specificity | ATPase(Phospho-Ser16)AntibodydetectsendogenouslevelsofATPaseonlywhenphosphorylatedatSer16. |
Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
Storage/StABIlity | Stableat-20°Cforatleast1year. |
NCBIGeneSymbol | ATP1A1 |
DatabaseLinks | GeneID: 476 Swiss-Prot#: P05023 OMIM#: 182310 Unigene#: Hs.371889 |
TargetModification | Phospho |
ModificationSites | H:S16M:S16R:S16 |
ApplicationImages |
Image1 | Immunohistochemistryanalysisofparaffin-embeddedhumanlungcarcinoma,usingATPase(Phospho-Ser16)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image2 | Westernblotanalysisoflysatesfrom293cellstreatedwithPMA125ng/ml30",usingATPase(Phospho-Ser16)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |