Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
| ProductName | Calnexin(Phospho-Ser583)Antibody |
| Synonyms | CALX;CANX;IP90;MajorhistocompatibilitycomplexclassI |
| Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanCalnexinaroundthephosphorylationsiteofSer583. |
| ImmunogenRange | 543-592 |
| Clonality | Polyclonal |
| ApplicationDilutions | WB:1:500~1:1000IHC:1:50~1:100IF:1:100~1:500ELISA:1:5000 |
| Specificity | Calnexin(Phospho-Ser583)AntibodydetectsendogenouslevelsofCalnexinonlywhenphosphorylatedatSer583. |
| Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
| PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
| Storage/StABIlity | Stableat-20°Cforatleast1year. |
| NCBIGeneSymbol | CANX |
| DatabaseLinks | GeneID: 821 Swiss-Prot#: P27824 OMIM#: 114217 Unigene#: Hs.699155 |
| TargetModification | Phospho |
| ModificationSites | H:S583M:S582R:S582 |
| ApplicationImages |
| Image1 | ImmunofluorescenceanalysisofHeLacells,usingCalnexin(Phospho-Ser583)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
| Image2 | Immunohistochemistryanalysisofparaffin-embeddedhumanbreastcarcinoma,usingCalnexin(Phospho-Ser583)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
| Image3 | WesternblotanalysisoflysatesfromHeLacellstreatedwithEGF200ng/ml30",usingCalnexin(Phospho-Ser583)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |