The antiserum was produced against synthesized peptide derived from human MLC-2 around the phosphorylation site of Thr17 and Ser18.
Immunogen Range
May-54
Clonality
Polyclonal
Application Dilutions
IHC: 1:50~1:100 ICC 1:50~100 ELISA: 1:10000
Specificity
MLC-2 (Phospho-Thr17+Ser18) Antibody detects endogenous levels of MLC-2 only when phosphorylated at Thr17 and Ser18.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.