The antiserum was produced against synthesized peptide derived from human NFAT4 around the phosphorylation site of Ser165.
Immunogen Range
131-180
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000
Specificity
NFAT4 (Phospho-Ser165) Antibody detects endogenous levels of NFAT4 only when phosphorylated at Ser165.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
NFATC3
Database Links
Gene ID: 4775 Swiss-Prot #: Q12968
OMIM #: 602698 Unigene #: Hs.632209
Target Modification
Phospho
Modification Sites
H:S165 M:S165
Application Images
Image 1
Immunofluorescence analysis of HeLa cells, using NFAT4 (Phospho-Ser165) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from HeLa cells treated with Ca+ 40nM 30', using NFAT4 (Phospho-Ser165) Antibody. The lane on the right is blocked with the phospho peptide.