The antiserum was produced against synthesized peptide derived from human PLCG1 around the phosphorylation site of Tyr1253.
Immunogen Range
1221-1270
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:20000
Specificity
PLCG1 (Phospho-Tyr1253) Antibody detects endogenous levels of PLCG1 only when phosphorylated at Tyr1253.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
PLCG1
Database Links
Gene ID: 5335 Swiss-Prot #: P19174
OMIM #: 172420 Unigene #: Hs.268177
Target Modification
Phospho
Modification Sites
H:Y1253 M:Y1253 R:Y1253
Application Images
Image 1
Western blot analysis of lysates from LOVO cells treated with and 293 cells treated with heat shock, using PLCG1 (Phospho-Tyr1253) Antibody. The lane on the right is blocked with the phospho peptide.