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| Note: The product is for research use only, not for use in diagnostic or therapeutic procedures |
|
| Product Name | ACK1 (Phospho-Tyr284) Antibody |
| Synonyms | ACK1; Activated p21cdc42Hs kinase; Non-receptor protein tyrosine kinase Ack; TNK2; kinase ACK1 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ACK1 around the phosphorylation site of Tyr284. |
| Immunogen Range | 250-299 |
| Clonality | Polyclonal |
| Application Dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 |
| Specificity | ACK1 (Phospho-Tyr284) Antibody detects endogenous levels of ACK1 only when phosphorylated at Tyr284. |
| Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
| Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage/Stability | Stable at -20°C for at least 1 year. |
| NCBI Gene Symbol | TNK2 |
| Database Links |
Gene ID: 10188 Swiss-Prot #: Q07912 OMIM #: 606994 Unigene #: Hs.518513 |
| Target Modification | Phospho |
| Modification Sites | H:Y284 M:Y284 |
| Application Images | |
| Image 1 |
 Immunofluorescence analysis of A549 cells, using ACK1 (Phospho-Tyr284) Antibody. The picture on the right is blocked with the phospho peptide. |
| Image 2 |
 Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ACK1 (Phospho-Tyr284) Antibody. The picture on the right is blocked with the phospho peptide. |
| Image 3 |
 Western blot analysis of lysates from HepG2 cells treated with EGF 200ng/ml 30', using ACK1 (Phospho-Tyr284) Antibody. The lane on the right is blocked with the phospho peptide. |
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