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| Note: The product is for research use only, not for use in diagnostic or therapeutic procedures |
|
| Product Name | Pim-1 (Phospho-Tyr309) Antibody |
| Synonyms | PIM-1; Proto-oncogene serine/threonine-protein kinase pim-1 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Pim-1 around the phosphorylation site of Tyr309. |
| Immunogen Range | 281-330 |
| Clonality | Polyclonal |
| Application Dilutions | WB: 1:500~1:1000 ELISA: 1:40000 |
| Specificity | Pim-1 (Phospho-Tyr309) Antibody detects endogenous levels of Pim-1 only when phosphorylated at Tyr309. |
| Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
| Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage/Stability | Stable at -20°C for at least 1 year. |
| NCBI Gene Symbol | PIM1 |
| Database Links |
Gene ID: 5292 Swiss-Prot #: P11309 OMIM #: 164960 Unigene #: Hs.81170 |
| Target Modification | Phospho |
| Modification Sites | H:Y309 M:Y302 R:Y218 |
| Application Images | |
| Image 1 |
 Western blot analysis of lysates from HUVEC cells treated with PMA 125ng/ml 30', using Pim-1 (Phospho-Tyr309) Antibody. The lane on the right is blocked with the phospho peptide. |
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