The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
PKD1/2/3/PKC mu (Phospho-Ser738+Ser742) Antibody
Synonyms
KPCD1; PKC-mu; PKCM; PKD; PRKCM; PRKD1; Protein kinase C, mu type; Protein kinase D; kinase PKD1; nPKC-mu
Immunogen
The antiserum was produced against synthesized peptide derived from human PKD1/2/3/PKC mu around the phosphorylation site of Ser738 and Ser742.
Immunogen Range
706-755
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
Specificity
PKD1/2/3/PKC mu (Phospho-Ser738+Ser742) Antibody detects endogenous levels of PKD1/2/3/PKC mu only when phosphorylated at Ser738 and Ser742.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunohistochemistry analysis of paraffin-embedded human heart, using PKD1/2/3/PKC mu (Phospho-Ser738+Ser742) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from A549 cells treated with PMA 125ng/ml 30' and HT29 cells treated with serum 20% 15', using PKD1/2/3/PKC mu (Phospho-Ser738+Ser742) Antibody. The lane on the right is blocked with the phospho peptide.