Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
| ProductName | PKD1/2/3/PKCmu(Phospho-Ser738+Ser742)Antibody |
| Synonyms | KPCD1;PKC-mu;PKCM;PKD;PRKCM;PRKD1;ProteinkinaseC,mutype;ProteinkinaseD;kinasePKD1;nPKC-mu |
| Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanPKD1/2/3/PKCmuaroundthephosphorylationsiteofSer738andSer742. |
| ImmunogenRange | 706-755 |
| Clonality | Polyclonal |
| ApplicationDilutions | WB:1:500~1:1000IHC:1:50~1:100ELISA:1:5000 |
| Specificity | PKD1/2/3/PKCmu(Phospho-Ser738+Ser742)AntibodydetectsendogenouslevelsofPKD1/2/3/PKCmuonlywhenphosphorylatedatSer738andSer742. |
| Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
| PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
| Storage/StABIlity | Stableat-20°Cforatleast1year. |
| NCBIGeneSymbol | KPCD1 |
| DatabaseLinks | GeneID: 5587/25865/23683 Swiss-Prot#: Q15139/Q9BZL6/O94806 OMIM#: 605435/607074/607077 Unigene#: Hs.508999/Hs.466987/Hs.660757/Hs.696257 |
| TargetModification | Phospho |
| ModificationSites | H:S738+S742M:S744+S748R:S475+S479 |
| ApplicationImages |
| Image1 | Immunohistochemistryanalysisofparaffin-embeddedhumanheart,usingPKD1/2/3/PKCmu(Phospho-Ser738+Ser742)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
| Image2 | WesternblotanalysisoflysatesfromA549cellstreatedwithPMA125ng/ml30"andHT29cellstreatedwithserum20%15",usingPKD1/2/3/PKCmu(Phospho-Ser738+Ser742)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |