The antiserum was produced against synthesized peptide derived from human SGK around the phosphorylation site of Ser78.
Immunogen Range
41-90
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
SGK (Phospho-Ser78) Antibody detects endogenous levels of SGK only when phosphorylated at Ser78.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
SGK1
Database Links
Gene ID: 6446 Swiss-Prot #: O00141
OMIM #: 602958 Unigene #: Hs.510078
Target Modification
Phospho
Modification Sites
H:S78 M:S78 R:S78
Application Images
Image 1
Western blot analysis of lysates from 293 cells treated with UV 15', using SGK (Phospho-Ser78) Antibody. The lane on the right is blocked with the phospho peptide.