The antiserum was produced against synthesized peptide derived from human AMPK beta1 around the phosphorylation site of Ser181.
Immunogen Range
147-196
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
AMPK beta1 (Phospho-Ser181) Antibody detects endogenous levels of AMPK beta1 only when phosphorylated at Ser181.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.