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| Note: The product is for research use only, not for use in diagnostic or therapeutic procedures |
|
| Product Name | B-RAF (Phospho-Thr599) Antibody |
| Synonyms | B-RAF; B-RAF proto-oncogene serine/threonine-protein kinase; B-Raf proto-oncogene serine/threonine-protein kinase; BRAF; BRAF1; c-RMIL; kinase B-Raf; p94; RAFB1; RMIL; RMIL serine/threonine-protein kinase; v-Raf murine sarcoma viral oncogene homolog B1 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human B-RAF around the phosphorylation site of Thr599. |
| Immunogen Range | 571-620 |
| Clonality | Polyclonal |
| Application Dilutions | IHC: 1:50~1:100 ELISA: 1:40000 |
| Specificity | B-RAF (Phospho-Thr599) Antibody detects endogenous levels of B-RAF only when phosphorylated at Thr599. |
| Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
| Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage/Stability | Stable at -20°C for at least 1 year. |
| NCBI Gene Symbol | BRAF1 |
| Database Links |
Gene ID: 673 Swiss-Prot #: P15056 OMIM #: 114500/115150/164757/211980/605027 Unigene #: Hs.550061 |
| Target Modification | Phospho |
| Modification Sites | H:T599 M:T636 R:T636 |
| Application Images | |
| Image 1 |
 Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using B-RAF (Phospho-Thr599) Antibody. The picture on the right is blocked with the phospho peptide. |
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