The antiserum was produced against synthesized peptide derived from human CDC25B around the phosphorylation site of Ser353.
Immunogen Range
319-368
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000
Specificity
CDC25B (Phospho-Ser353) Antibody detects endogenous levels of CDC25B only when phosphorylated at Ser353.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
CDC25A
Database Links
Gene ID: 994 Swiss-Prot #: P30305
OMIM #: 116949 Unigene #: Hs.437705
Target Modification
Phospho
Modification Sites
H:S353
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human brain, using CDC25B (Phospho-Ser353) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from COS7 cells treated with etoposide 25uM 24h, using CDC25B (Phospho-Ser353) Antibody. The lane on the right is blocked with the phospho peptide.