Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
| ProductName | CDC25B(Phospho-Ser353)Antibody |
| Synonyms | CDC25HU2;CDC25M2;DualspecificityphosphataseCdc25B;M-phaseinducerphosphatase2;MPIP2 |
| Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanCDC25BaroundthephosphorylationsiteofSer353. |
| ImmunogenRange | 319-368 |
| Clonality | Polyclonal |
| ApplicationDilutions | WB:1:500~1:1000IHC:1:50~1:100ELISA:1:40000 |
| Specificity | CDC25B(Phospho-Ser353)AntibodydetectsendogenouslevelsofCDC25BonlywhenphosphorylatedatSer353. |
| Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
| PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
| Storage/StABIlity | Stableat-20°Cforatleast1year. |
| NCBIGeneSymbol | CDC25A |
| DatabaseLinks | GeneID: 994 Swiss-Prot#: P30305 OMIM#: 116949 Unigene#: Hs.437705 |
| TargetModification | Phospho |
| ModificationSites | H:S353 |
| ApplicationImages |
| Image1 | Immunohistochemistryanalysisofparaffin-embeddedhumanbrain,usingCDC25B(Phospho-Ser353)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
| Image2 | WesternblotanalysisoflysatesfromCOS7cellstreatedwithetoposide25uM24h,usingCDC25B(Phospho-Ser353)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |