Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
| ProductName | Keratin18(Phospho-Ser52)Antibody |
| Synonyms | CK18;CK18;CYK18;CytokeratinendoB;K18;K1C18;KERD;KRT1-18;KRT18;Keratin;KeratinD;Keratin;typeIcytoskeletal18;cytokeratin18;typeIcytoskeletal18 |
| Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanKeratin18aroundthephosphorylationsiteofSer52. |
| ImmunogenRange | 21-70 |
| Clonality | Polyclonal |
| ApplicationDilutions | WB:1:500~1:1000IF:1:100~1:500ELISA:1:10000 |
| Specificity | Keratin18(Phospho-Ser52)AntibodydetectsendogenouslevelsofKeratin18onlywhenphosphorylatedatSer52. |
| Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
| PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
| Storage/StABIlity | Stableat-20°Cforatleast1year. |
| NCBIGeneSymbol | KRT18 |
| DatabaseLinks | GeneID: 3875 Swiss-Prot#: P05783 OMIM#: 148070/215600 Unigene#: Hs.406013 |
| TargetModification | Phospho |
| ModificationSites | H:S52 |
| ApplicationImages |
| Image1 | ImmunofluorescenceanalysisofHeLacells,usingKeratin18(Phospho-Ser52)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
| Image2 | WesternblotanalysisoflysatesfromHepG2cells,usingKeratin18(Phospho-Ser52)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |