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| Note: The product is for research use only, not for use in diagnostic or therapeutic procedures |
|
| Product Name | Smad2 (Phospho-Thr220) Antibody |
| Synonyms | JV18-1; MADH2; MADR2; Mad-related protein 2; Mothers against DPP homolog 2; Mothers against decapentaplegic homolog 2; Smad 2 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Smad2 around the phosphorylation site of Thr220. |
| Immunogen Range | 186-235 |
| Clonality | Polyclonal |
| Application Dilutions | IF: 1:100~1:500 ELISA: 1:1000 |
| Specificity | Smad2 (Phospho-Thr220) Antibody detects endogenous levels of Smad2 only when phosphorylated at Thr220. |
| Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
| Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage/Stability | Stable at -20°C for at least 1 year. |
| NCBI Gene Symbol | SMAD2 |
| Database Links |
Gene ID: 4087 Swiss-Prot #: Q15796 OMIM #: 601366 Unigene #: Hs.12253/Hs.705764 |
| Target Modification | Phospho |
| Modification Sites | H:T220 M:T220 R:T220 |
| Application Images | |
| Image 1 |
 Immunofluorescence analysis of HeLa cells, using Smad2 (Phospho-Thr220) Antibody. The picture on the right is blocked with the phospho peptide. |
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