Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
ProductName | Smad2/3(Phospho-Thr8)Antibody |
Synonyms | JV15-2;MAD-3;MADH3;Mad3;MothersagainstDPPhomolog3;Mothersagainstdecapentaplegichomolog3;SMAD3;Smad3 |
Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanSmad2/3aroundthephosphorylationsiteofThr8. |
ImmunogenRange | Jan-50 |
Clonality | Polyclonal |
ApplicationDilutions | WB:1:500~1:1000ELISA:1:10000 |
Specificity | Smad2/3(Phospho-Thr8)AntibodydetectsendogenouslevelsofSmad2/3onlywhenphosphorylatedatThr8. |
Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
Storage/StABIlity | Stableat-20°Cforatleast1year. |
NCBIGeneSymbol | SMAD3 |
DatabaseLinks | GeneID: 4088/4087 Swiss-Prot#: P84022/Q15796 OMIM#: 114500/603109/601366 Unigene#: Hs.618504/Hs.714621/Hs.12253/Hs.705764 |
TargetModification | Phospho |
ModificationSites | H:T8M:T8R:T8 |
ApplicationImages |
Image1 | WesternblotanalysisoflysatesfromRAW264.7cells,usingSmad2/3(Phospho-Thr8)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |