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| Note: The product is for research use only, not for use in diagnostic or therapeutic procedures |
|
| Product Name | Smad3 (Phospho-Ser204) Antibody |
| Synonyms | JV15-2; MAD-3; MADH3; Mad3; Mothers against DPP homolog 3; Mothers against decapentaplegic homolog 3; SMAD 3; Smad 3 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Smad3 around the phosphorylation site of Ser204. |
| Immunogen Range | 170-219 |
| Clonality | Polyclonal |
| Application Dilutions | WB: 1:500~1:1000 ELISA: 1:20000 |
| Specificity | Smad3 (Phospho-Ser204) Antibody detects endogenous levels of Smad3 only when phosphorylated at Ser204. |
| Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
| Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage/Stability | Stable at -20°C for at least 1 year. |
| NCBI Gene Symbol | SMAD3 |
| Database Links |
Gene ID: 4088 Swiss-Prot #: P84022 OMIM #: 114500/603109 Unigene #: Hs.618504/Hs.714621 |
| Target Modification | Phospho |
| Modification Sites | H:S204 M:S204 R:S204 |
| Application Images | |
| Image 1 |
 Western blot analysis of lysates from NIH/3T3 cells treated with Serum 20% 15', using Smad3 (Phospho-Ser204) Antibody. The lane on the right is blocked with the phospho peptide. |
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