Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
ProductName | IRAK1(Phospho-Thr100)Antibody |
Synonyms | IL1RAK;IRAK;IRAK-1;Interleukin-1receptor-associatedkinase1;Pelle-likeproteinkinase;kinaseIRAK1;mPLK |
Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanIRAK1aroundthephosphorylationsiteofThr100. |
ImmunogenRange | 66-115 |
Clonality | Polyclonal |
ApplicationDilutions | WB:1:500~1:1000IHC:1:50~1:100ELISA:1:20000 |
Specificity | IRAK1(Phospho-Thr100)AntibodydetectsendogenouslevelsofIRAK1onlywhenphosphorylatedatThr100. |
Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
Storage/StABIlity | Stableat-20°Cforatleast1year. |
NCBIGeneSymbol | IRAK1 |
DatabaseLinks | GeneID: 3654 Swiss-Prot#: P51617 OMIM#: 300283 Unigene#: Hs.200716/Hs.522819 |
TargetModification | Phospho |
ModificationSites | H:T100M:T100R:T100 |
ApplicationImages |
Image1 | Immunohistochemistryanalysisofparaffin-embeddedhumancoloncarcinoma,usingIRAK1(Phospho-Thr100)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image2 | WesternblotanalysisoflysatesfromJurkatcellstreatedwithheatshock,usingIRAK1(Phospho-Thr100)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |