The antiserum was produced against synthesized peptide derived from human IRAK1 around the phosphorylation site of Thr209.
Immunogen Range
175-224
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:40000
Specificity
IRAK1 (Phospho-Thr209) Antibody detects endogenous levels of IRAK1 only when phosphorylated at Thr209.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
IRAK1
Database Links
Gene ID: 3654 Swiss-Prot #: P51617
OMIM #: 300283 Unigene #: Hs.200716/Hs.522819
Target Modification
Phospho
Modification Sites
H:T209
Application Images
Image 1
Western blot analysis of lysates from HeLa cells, using IRAK1 (Phospho-Thr209) Antibody. The lane on the right is blocked with the phospho peptide.