The antiserum was produced against synthesized peptide derived from human ITK around the phosphorylation site of Tyr512.
Immunogen Range
478-527
Clonality
Polyclonal
Application Dilutions
IHC: 1:50~1:100 ELISA: 1:10000
Specificity
ITK (Phospho-Tyr512) Antibody detects endogenous levels of ITK only when phosphorylated at Tyr512.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.