Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
ProductName | Kv1.3/KCNA3(Phospho-Tyr135)Antibody |
Synonyms | CIK3;HGK5;HLK3;HPCN3;HuKIII;KCNA3;PotassiumchannelKv1.3;Potassiumvoltage-gatedchannelsubfamilyAmember3 |
Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanKv1.3/KCNA3aroundthephosphorylationsiteofTyr135. |
ImmunogenRange | 101-150 |
Clonality | Polyclonal |
ApplicationDilutions | WB:1:500~1:1000IHC:1:50~1:100IF:1:100~1:500ELISA:1:20000 |
Specificity | Kv1.3/KCNA3(Phospho-Tyr135)AntibodydetectsendogenouslevelsofKv1.3/KCNA3onlywhenphosphorylatedatTyr135. |
Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
Storage/StABIlity | Stableat-20°Cforatleast1year. |
NCBIGeneSymbol | KCNA3 |
DatabaseLinks | GeneID: 3738 Swiss-Prot#: P22001 OMIM#: 176263 Unigene#: Hs.169948 |
TargetModification | Phospho |
ModificationSites | H:Y135M:Y140R:Y137 |
ApplicationImages |
Image1 | ImmunofluorescenceanalysisofHUVECcells,usingKv1.3/KCNA3(Phospho-Tyr135)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image2 | Immunohistochemistryanalysisofparaffin-embeddedhumanbrain,usingKv1.3/KCNA3(Phospho-Tyr135)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image3 | WesternblotanalysisoflysatesfromJurkatcellstreatedwithstarved24h,usingKv1.3/KCNA3(Phospho-Tyr135)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |