Description
SILACAdvancedDMEM/F-12FlexisAdvancedDMEM/F-12withoutL-arginine,L-glutamine,andL-lysineforuseduringSILACproteinlabelingwithstableisotopiclabeledlysineand/orarginine.AdvancedDMEM/F-12isawidelyusedbasalmediumforsupportingthegrowthofmanydifferentmammaliancells,whichreducestheFBSrequirementsby50-90%withoutanychangesingrowthrateormorphology.Whensupplementedwith1-2%FBS,AdvancedDMEM/F-12Flexiscapableofsupportingcellularproliferationandmaximumcelldensitiescomparabletotheconventionalbasalformulationsupplementedwith5-10%FBS.CellssuccessfullyculturedinAdvancedDMEM/F-12withnoadaptationincludeJurkat,WI-38,SP2,Vero,andMRC-5.WeofferavarietyofDMEM/F-12modificationsforarangeofapplications.Findtherightformulationusingthemediaselectortool.
ThisAdvancedDMEM/F-12ismanufacturedasfollows:| With | Without | 
| •Non-essentialaminoacids | •Glucose | 
| •Sodiumpyruvate | •Phenolred | 
|  | •L-arginine | 
|  | •L-glutamine | 
|  | •L-lysine | 
|  | •HEPES | 
 AdvancedDMEM/F-12isuniquefromothermediaduetoadditionofthefollowingingredientstoallowforserumreduction:ethanolamine,glutathione,ascorbicacid,insulin,transferrin,AlbuMAX®Ilipid-richbovineserumalbuminforcellculture,andthetraceelementssodiumselenite,ammoniummetavanadate,cupricsulfate,andmanganouschloride.AdvancedDMEM/F-12typicallyrequiressupplementationwith1-5%FetalBovineSerumand4mML-glutamineorGlutaMAX™supplement(optional).SILACproteinlabelingexperimentsshouldbeconductedusing
Gibco™DialyzedFBS.TheFBSconcentrationmustbeoptimizedforeachcelllinetoobtainmaximumserumreduction.AdvancedDMEM/F-12usesasodiumbicarbonatebuffersystem(3.7g/L),andthereforerequiresa5-10%CO
2environmenttomaintainphysiologicalpH.
cGMPManufacturingandQualitySystemSILACAdvancedDMEM/F-12FlexismanufacturedatacGMP-compliantfacilitylocatedinGrandIsland,NewYork.ThefacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtoISO13485standards.
ForResearchUseorFurtherManufacturing.Notforuseindiagnosticprocedures.