The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
G3BP-1 (Phospho-Ser232) Antibody
Synonyms
Ras GTPase-activating protein-binding protein 1; G3BP-1; ATP-dependent DNA helicase VIII; HDH-VIII; GAP SH3 domain-binding protein 1; G3BP1; G3BP
Immunogen
The antiserum was produced against synthesized peptide derived from human G3BP-1 around the phosphorylation site of Ser232.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
Specificity
G3BP-1 (Phospho-Ser232) Antibody detects endogenous levels of G3BP-1 only when phosphorylated at Ser232.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Store at -20°C/1 year
NCBI Gene Symbol
G3BP1
Database Links
Gene ID: 10146 Swiss-Prot #: Q13283
OMIM #: 608431 Unigene #: Hs.587054
Target Modification
Phospho
Modification Sites
H:S232 M:S231
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using G3BP-1 (Phospho-Ser232) Antibody. The picture on the right is treated with the synthesized peptide.
Image 2
Western blot analysis of extracts from HeLa cells, using G3BP-1 (Phospho-Ser232) Antibody. The lane on the right is treated with the synthesized peptide.