The antiserum was produced against synthesized peptide derived from human HDAC5 around the phosphorylation site of Ser498.
Immunogen Range
464-513
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Specificity
HDAC5 (Phospho-Ser498) Antibody detects endogenous levels of HDAC5 only when phosphorylated at Ser498.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
HDAC5
Database Links
Gene ID: 10014 Swiss-Prot #: Q9UQL6
OMIM #: 605315 Unigene #: Hs.438782
Target Modification
Phospho
Modification Sites
H:S498 M:S488
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HDAC5 (Phospho-Ser498) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from Jurkat cells, using HDAC5 (Phospho-Ser498) Antibody. The lane on the right is blocked with the phospho peptide.